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Re: Cell boundary program? [message #21116 is a reply to message #21096]

Mon, 07 August 2000 00:00

Ben Tupper
Messages: 186
Registered: August 1999

Senior Member

Hello,

There are two methods you can use, convolution or contouring.

Each has its advantages. The contouring method is quick, but it doesn't return
the location of every pixel that makes up the cell boundary. You're best bet to
contour a binarized version of the image.

The other method, convolution is also quick, but the returned indices are in
scan-line order. In this case, all the boundary pixels are included. This
method was suggested by ... uhoh, I forgotten who it was right now, sorry.
Here's the steps as described on the newsgroup by the unknown author.

bb = convol(Image, replicate(1,3,3),9,/center)
Edges = Where(bb gt 0 AND bb LT 255)
bb[*] = 0
bb[edges] = 255 ; make this new image just edges
bb = Image AND BB ; now keep just those edges inside your object
perimeter = where(bb eq 255); these are indices to final outline

Ben

Alexandros Pertsinidis wrote:

> I am looking for an IDL program that determines the boundary of a cell. I
> have a lot of video of cells (neurons). Ideally, I would like track the
> change in their shape/size as time progresses. Does anyone know of anything
> like this? Thank you very much!
>
> Rachel

--
Ben Tupper
Pemaquid River Company
248 Lower Round Pond Road
POB 106
Bristol, ME 04539

Tel: (207) 563-1048
Email: PemaquidRiver@tidewater.net

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[Message index]

		Re: Cell boundary program? By: Rick Towler on Wed, 09 August 2000 00:00
		Re: Cell boundary program? By: Rick Towler on Tue, 08 August 2000 00:00
		Re: Cell boundary program? By: Alexandros Pertsinidi on Tue, 08 August 2000 00:00
		Re: Cell boundary program? By: Richard Adams on Tue, 08 August 2000 00:00
		Re: Cell boundary program? By: Ben Tupper on Mon, 07 August 2000 00:00

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